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BOFIN Insight Issue 1

Cracking the code for ‘slug-resistant’ wheat

Scientists are making progress towards finding what makes some wheat varieties resistant to slugs – potentially offering sustainable solutions to slug control.

One area of focus for scientists involved in the SLIMERS project is identifying what makes some varieties of wheat more ‘slug resistant’ than others. Research at the John Innes Centre is based on previous studies of the Centre’s Watkins collection of landrace wheat varieties which identified Watkins 788 as potentially ‘slug resistant’.

Dr Simon Griffiths crossed Watkins 788 with other varieties to create 77 Recombinant Inbred Lines (RILs) for further testing to help identify the gene or genes responsible for slug resistance. The centre’s Head of Entomology and Insectary Platform Dr Victor Soria-Carrasco is running trials on these RILs using slugs provided by the ‘Slug Scouts’ volunteers who capture grey field slugs in their farms or gardens and post them to the centre.

Three slugs are added to each tray with a selection of pre-soaked seeds and then left for two days. Seeds are weighed before and after the trial period and also scored for levels of damage before being left for a further seven days to assess the impact of the slug damage on germination.

Trial replications revealed RILs that appear to be slug resistant, as well as those that are more susceptible. Dr Soria-Carrasco and team will continue to test RILs further in the lab to help identify the part of the plant DNA associated with slug resistance – the quantitative trait locus (QTL).

“Detecting which wheat the slugs like is as relevant as finding the varieties they don’t like, as it will all help us identify the QTLs,” says Dr Soria-Carrasco.

Meanwhile two of the RILs – one which is susceptible and one showing resistance – plus Watkins 788 have been multiplied up for on-farm testing in 2024-5.

Tom Allen-Stevens says: “Seeing how the RILs perform in the field is the crucial next stage. We have six farmers establishing blocks of Watkins 788 and the two RILs, alongside farm standard wheat this autumn. They will take measurements and samples to fully assess whether they are indeed spurned by slugs and could offer a potential solution to farmers.”

SLIMERS is a three-year £2.6m research programme involving more than 100 UK farms and seven partners. SLIMERS is funded by the Small R&D Partnership Projects, part of Defra’s Farming Innovation Programme which is delivered by Innovate UK. Project number: 10053286.

Visit slimers.co.uk to find out more.

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BOFIN Insight Issue 1

Reducing soya imports has ‘never been so important’

A new event organised by partners in the NCS Project is setting out to drive the transition to a more sustainable, resilient and secure food system in the UK through reduced dependency on imported soya. ‘From Soya to Sustainability’ will be held on January 22, 2025 at KingsGate Conference Centre, Peterborough. It will bring together farmers, processors, manufacturers, researchers, policymakers and industry leaders to explore innovative strategies for integrating beans and other pulses into livestock diets, reducing the need for imported soya.

Headline speaker Philip Lymbery will emphasise the urgent need for change in our food systems. Philip is Global Chief Executive of Compassion in World Farming and author of Sixty Harvests Left: How to Reach a Nature-Friendly Future with other roles including visiting Professor at the University of Winchester.

Philip says: “I am delighted to be speaking at this new event which is setting out to drive the transition to a more sustainable, resilient and secure food system in the UK through reduced dependency on imported soya.

“It has never been more important to reduce soya imports for animal feed. Vast areas of rainforest and savannah in Brazil and Argentina are cleared essentially to grow soya to feed industrially reared animals, not least in the UK and Europe. The consequences are devastating to wild animals and the environment.

“There are much more compassionate, environmentally friendly, and sustainable ways to produce food, and I am looking forward to exploring these with participants at the conference.”

Other speakers will include experts leading the charge for reduced use of soya in the UK who will share the latest research and innovations in the production and use of peas and beans.

There will also be ample networking opportunities enabling delegates to connect with like-minded experts from across the supply chain. Roger Vickers of PGRO and NCS Project lead says:

“This event is a call to action for all in the agri-food supply chain to play their part in the move from soya to sustainability, which is urgently needed if we are to reduce our environmental impact.

“Growing more pulse crops in the UK and using them in livestock feed would help tackle our dependency on imported soya while also promoting agricultural practices that benefit farmers and the environment. There are multiple wins, but it is not an easy fix.

“We need urgent and concerted action across the supply chain if we are to make a difference. I encourage anyone working in the agri-food supply chain to attend and be part of a movement for change.”

The NCS project is a four-year £5.9m research programme led by PGRO with 17 partners. It aims to enable UK agriculture to bring about a reduction of 1.5Mt CO2e per annum through increased pulse and legume cropping. The ambition is to increase pulse and legume cropping in arable rotations to 20% across the UK from the current 5% and to replace 50% of imported soya meal used in livestock feed rations with home-grown legumes. The NCS Project is funded by the Farming Futures R&D Fund: Climate smart farming, part of Defra’s Farming Innovation Programme, which is delivered by Innovate UK. Project number 10043778.

Visit ncsproject.co.uk to find out more.

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BOFIN Insight Issue 1

Turning Witchcraft into Wizardry

On the Isle of Wight, Rachel and Jacob Holmes are keen that science steers the course on their journey into regenerative agriculture.

At the side of the yard are three IBC containers. The plastic tanks have been removed, and in their place is a black semi-permeable membrane, with each container about half full of what looks like compost. But it’s the four-inch diameter terracotta-coloured drainage pipes protruding from the IBCs that denote their purpose.

“The key requirement of a Johnson-Su bioreactor is that the compost must be kept aerobic,” explains Rachel Holmes.

Cable ties keep the pipes no more than 30cm apart, while holes drilled down their length keep the bacteria, fungi and other microbes multiplying in the compost.

The aim for the microbes in the bioreactor is to breathe new life into the loam over brash soils of the 340ha arable farm Rachel farms with her son Jacob on the Isle of Wight. The farm business, Independent Farms, has set a course on regenerative agriculture, reducing cultivations and introducing cover crops into a rotation that includes wheat, wholecrop rye and oilseed rape with additional breaks of stewardship legume fallows.

“The soils have been very intensely farmed over the years,” says Rachel. “The turning point was the drought of 2022 – cover crops simply didn’t germinate, and you just wonder whether there’s enough life in the soil to pull it through periods of more extreme weather.”

She takes a trowel and, leaning over the side of an IBC, doesn’t have to dig too far to find several worms, helping the process of breaking down the raw materials into a rich soil-like matter with an abundance of microbes. “There’s no question there’s life in this compost – Jacob has confirmed that by taking regular samples and studying them under a microscope,” continues Rachel.

“The big unknowns are whether we are being successful with transferring them into the field, and the difference they’re making to the health of the soil and the crop roots within it.”

This is why Rachel has joined as a Root Ranger with the TRUTH project. Independent Farms is one of 20 farms across the UK conducting crop trials and taking intensive soil samples to understand the microbial community within it, and the effect of applications of products or mixes claimed to be beneficial.

For Rachel and Jacob it’s the compost ‘tea’ they produce that’s under scrutiny. This starts its life in the Johnson-Su bioreactor – a DIY kit developed by Dr David Johnson, a molecular biologist at the University of New Mexico, and his wife, Hui-Chun Su. The compost created in this set-up is then brewed to produce an extract which is sprayed on the land or can be applied to seed.

Rachel explains that the key to the process is finding the right mix of raw materials for the bioreactor. “We’re keen to use material found locally and have also added spent coffee granules from a local café. It’s quite addictive – once you get started you get hooked on finding the right recipe.”

A tag on the side of this IBC reveals woodchip, manure and coffee are the ingredients being turned over by the worms. In the next-door container hay has been added, while a mix of hay, fresh-cut grass, manure and chopped rye complete the trio.

The mix can spend over a year going through its process before it’s ready, and while it doesn’t need to be turned, like regular compost, it should be monitored and kept moist. Just to the side, in the yard, there are two containers of vermicast. “It’s essentially worm poo,” explains Rachel.

“You can buy it, but ours is home-grown from kitchen scraps. A bag each of the vermicast and compost are dropped into the brewing container, like two giant tea bags.”

Wandering over to the spray shed, another set of IBCs, this time with plastic tank intact, form the brewing set-up, and Jacob talks through the process. “The bags are a 400-micron mesh, that can let microbes as large as nematodes through, but not the residue. Each 1000-litre tank is filled with water and we add one litre of Actiferm, a liquid mix of fungi, yeast, lactic acid bacteria, phototropic bacteria and actinobacteria, along with a dollop of molasses.

“Air is then bubbled through the tank for 24 hours and it’s ready to apply. That’s the tricky bit – you have to be sure you can spray at the right time or the mixture will go past its best. Extract with twice as much bacteria as fungi is the ideal, and according to the literature, an application of around 200l/ha puts down 60 million bacteria and fungi per square metre.”

Jacob has a voracious appetite for information on all aspects of soil biology he’s gleaned from the likes of Dr Elaine Ingham, the webinars he’s attended and contacts he’s made. “Living here on the Isle of Wight it can be difficult to get to events geared towards understanding soil biology. But we’ve been to Groundswell and the Green Farm Collective event – both of these have proven useful, especially to hear other farmers discuss what they’re doing,” he says.

He collates this all into a referenced document that helps him understand the microbes he monitors using his microscope. Much of the data has also been brought into a handy app he’s developed that aids the somewhat complex calculations he makes to ensure the right balance of microbes in the extract brewed and applied to the fields.

“Preparing and applying biology to the soil through a sprayer is totally different to using chemistry as it’s a living material that changes by the hour. For instance, bacteria multiply in the brewed extract every 20 minutes, fungi typically every 4-6 hours, protozoans multiply threefold in 24 hours, while that’s too short a timespan for nematodes to multiply. But maintaining the right balance is crucial, as they all have different tasks and respond differently to environmental factors in the field.”

And it’s this response in the field that has been the missing piece of the jigsaw. Jacob has gone to admirable lengths to explore the microbiome he can introduce to the soil, its interaction with the specific environmental factors it faces, and the issues with bringing it, in the right balance, to where it can get to work.

“This is a brand-new school of science we’re just beginning to learn,” he enthuses. “There are so many living organisms in the soil and in our attempts to harness them, we’re not sure whether we’re creating brown gold or doing a sh** job of being King Midas. The science is good, but without results it’s just witchcraft. Anything we can do to fill that void will help turn the witchcraft into wizardry.”

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BOFIN Insight Issue 1

Root Rangers to trial ‘remarkable’ wheat

Thriving Roots Underpinning Total soil Health (TRUTH) is creating a farmer-led platform to quantify and evaluate soil/root interactions and explore the capabilities of a novel sensor that can ‘smell’ a soil’s biological signature.

Farmers in the TRUTH project will soon be trialling a wheat variety that can moderate its own nitrogen supply. Originating from Iran, this particular wheat landrace is part of the historic Watkins collection at the John Innes Centre in Norwich where research scientist Dr Maria Hernandez-Soriano discovered it had some special abilities.

She explains: “What is interesting about this particular wheat, is that it has demonstrated capacity to decrease the transformation of ammonium into nitrate in the soil. This is believed to be an adaptive trait.

“Some wheat cultivars have evolved to put compounds in the soil that can help the plant uptake nitrogen from soils where this essential nutrient is not particularly abundant.”

By slowing down activity of soil microbes that quickly transform ammonium into nitrate it allows the plant to make better use of ammonium and nitrogen in the soil. This means less nitrogen from fertilisers potentially being lost to the environment and more being taken up and used by the plant.

Maria and fellow researchers have identified where in the wheat’s genome the capability to control these microbes is. They are now working on identifying the specific compound responsible for its nitrogen- monitoring abilities. These types of compounds have been identified in some sorghum, rice and wild grasses.

“We haven’t observed that same capability or trait in any commercial wheat cultivars yet, so that is remarkable,” Maria continues.

Seeds of these promising wheat cultivars have been multiplied at the centre’s research farm near Norwich and will be distributed to farmers working on the TRUTH project.

It is one of several varieties that the 20 Root Rangers will be growing in 1ha plots alongside farm standard varieties. Trial plots will be thoroughly tested using metagenomics and soil nitrification measurements.

“This means we can prove that there is not only suppression or control of the nitrifying microbes, but a suppression of the action,” Maria concludes.

Comparing the roots of this variety to those of farm standard wheat cultivars should therefore help quantify the impact of the Watkins cultivar on microbial action, and ultimately prove (or disprove) its ability to moderate its own nitrogen supply.

TRUTH is a three-year £1m research programme, led by BOFIN alongside four partners. Launched in 2024 it is funded by the Farming Futures R&D fund, part of Defra’s Farming Innovation Programme which is delivered by Innovate UK. Project number: 10085980.

Visit bofin.org.uk/truthproject to find out more.

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BOFIN Insight Issue 1

Will you take the #PROBITYPledge?

Agriculture is under pressure to increase production while also mitigating and responding to climate change. Innovative approaches such as precision breeding are therefore becoming increasingly important.

A Platform to Rate Organisms Bred for Improved Trait and Yield (PROBITY) will see precision-bred crop varieties being trialled and scrutinised by farmers, scientists and food manufacturers in an open and transparent forum.

A new initiative invites farmers and others from across the industry to engage in a nationwide discussion on precision bred crops and their potential impact on UK agriculture.

As the Government moves forward with legislation to support precision breeding, the #PROBITYPledge campaign will provide farmers with accurate information and a platform to voice their opinions.

Precision-bred crops can be created through gene editing, a widely used technology that enables scientists to make changes to plant DNA, like those that occur naturally, but introduced in a precise, targeted manner. Gene editing speeds up the process of breeding more productive, nutritional and sustainable crop varieties.

The #PROBITYPledge campaign asks farmers to pledge a minimum of one hour in the next six months, to learn about precision breeding and share views via The Sequence Circle online forum.

Tom Allen-Stevens says: “This is a topic which will have an enormous impact on sustainable food and farming, so it’s important to make time to discuss it.

“As farmers we are the gatekeepers of precision-breeding technology and must lead the discussion on how it is introduced to create a trusted pathway for food manufacturers to deliver its potential benefits to consumers.

“We want to hear diverse views – what do you think, what are your hopes and fears are for precision breeding, and what questions do you have about gene editing?

“Our Sequence Circle community hosted on The Farming Forum includes some incredible scientists who are well placed to help answer those questions.”

Anyone with an interest in the future or food and farming is welcome to make the #PROBITYPledge, sign up and get involved with learning and discussing this technology, he added.

Contributions to The Sequence Circle hosted on The Farming Forum will be used (anonymously) by researchers, so those signing up need to be over 18 years of age.

PROBITY is a three-year £2.2m farmer-led project with 12 research and industry partners. It is funded by Defra’s Farming Innovation Programme which is delivered by Innovate UK. Project number: 10097973.

Visit probityproject.co.uk to find out more.